Key facts
- Brand
- Agilent
- Model
- 7900HT
- Industry
- Industrial
- Documents
- 5
- Languages
- EN
- Last added
- 2012-07-04
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Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix - Quick Reference Guide for the ABI 7900HT Fast Real-Time PCR System Manual
This document serves as a quick reference guide for preparing reactions and setting up experiments using the Stratagene Brilliant III Ultra-Fast SYBR Green QPCR Master Mix. It details the steps to dilute the reference dye, prepare reagent mixtures with specific primer concentrations (200–500 nM), and add experimental DNA to achieve a final reaction volume of 20 μl.
Read onlineAgilent 7900HT specifications
| Final reaction volume | 20 μl |
|---|---|
| Primer concentration range | 200–500 nM |
| Reference dye dilution ratio | 1:50 |
| Number of reactions (Catalog #600805) | 400 reactions |
All Agilent 7900HT documents by type
The shortest path to a working setup, usually only a few pages.
- Agilent Brilliant III Ultra-Fast SYBR Green QPCR Master Mix - Quick Reference Guide for the ABI 7900HT Fast Real-Time PCR System Manual 2 p
- Agilent Technologies Brilliant III Ultra-Fast QRT-PCR Master Mix: Quick Start Guide for the ABI 7900HT Fast Real-Time PCR System 2 p
- Agilent Technologies Brilliant III Ultra-Fast QPCR Master Mix: Quick Start Guide for the ABI 7900HT Fast Real-Time PCR System
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Ratings, dimensions and ordering codes, for selection and design work.
Questions answered in these manuals
What is the recommended final reaction volume?
The guide recommends bringing the final reaction volume to 20 μl.
How should the reference dye be prepared before use?
It must be diluted 1:50 using nuclease-free PCR-grade water.
What instrument is this master mix designed for?
It is designed for the ABI 7900 HT Real-Time PCR instrument.
From: Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for ABI 7900 HT Data Sheet
Does this master mix support SYBR Green chemistry?
Yes, it is compatible with sequence-specific probes and SYBR® Green dyes.
From: Agilent Brilliant III Ultra-Fast QPCR/QRT-PCR Master Mixes for ABI 7900 HT Data Sheet
What is the final reaction volume?
The final reaction volume should be brought to 20 μl.
How much diluted reference dye is required per reaction?
You need to add 0.3 μl of diluted reference dye.