This quick reference guide provides an optimized protocol for using the Stratagene Brilliant III Ultra- Fast SYBR® Green QPCR Master Mix with the 7900HT Fast Real-Time PCR System from Applied Biosystems. For detailed instructions, refer to the full product manual.
This document serves as a quick reference guide for preparing reactions and setting up experiments using the Stratagene Brilliant III Ultra-Fast SYBR Green QPCR Master Mix. It details the steps to dilute the reference dye, prepare reagent mixtures with specific primer concentrations (200–500 nM), and add experimental DNA to achieve a final reaction volume of 20 μl.
| Final reaction volume | 20 μl |
|---|---|
| Primer concentration range | 200–500 nM |
| Reference dye dilution ratio | 1:50 |
| Number of reactions (Catalog #600805) | 400 reactions |
The guide recommends bringing the final reaction volume to 20 μl.
It must be diluted 1:50 using nuclease-free PCR-grade water.
The guide suggests an optimized concentration of 200–500 nM for both upstream and downstream primers.