Size-exclusion chromatography is a powerful technique for the size-dependent separation of biomolecules. While standard, non-denaturing mobile phases are often desirable, denaturing mobile phases containing guanidine hydrochloride (or SDS), may be used to insure un-aggregated, monomers for proper size determination. As shown below, denaturation shifts molecules toward larger apparent size and smaller retention volume.
The document discusses size-exclusion chromatography (SEC) as a technique for separating biomolecules based on size. It highlights that while standard non-denaturing mobile phases are common, denaturing phases containing guanidine hydrochloride or SDS can be used to ensure un-aggregated monomers for accurate size determination.
| Column Part Number | 884973-902 |
|---|---|
| Molecular Weight Range | 10,000-1,000,000 daltons |
| Flow Rate | 2.0 mL / min |
The column separates biomolecules within a molecular-weight range of 10,000 to 1,000,000 daltons.
The column has dimensions of 9.4 x 250 and Agilent Part No. 884973-902.