Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview

The paper introduces the characteristics of superficially porous particles and discusses the transfer of methods from larger particle columns (5-µm) to superficially porous particle columns (120 EC-C18) and sub-2-µm totally porous columns (ZORBAX Eclipse Plus C18)

Brand: Agilent
Document type: Product Leaflet
Language: English
Pages: 14
File format: PDF
File size: 931 KB
Published: 05 July, 2012
Updated: 30 September, 2023
Models: EC-C18, Poroshell 120 EC-C18, Poroshell 120, ZORBAX Eclipse Plus C18, Eclipse Plus C18
MD5 checksum: 9001B8D788059E155F0F4B6D942560DA
PDF: Agilent Poroshell 120 EC-C18 and ZORBAX Eclipse Plus C18 Method Transfer Technical Overview PDF

About this manual

This document provides a technical overview of transferring chromatographic methods from larger totally porous particles to superficially porous particles, specifically comparing Agilent Poroshell 120 EC-C18 and Agilent ZORBAX Eclipse Plus C18 columns. It highlights the benefits of using superficially porous particles for time savings and reduced backpressure while maintaining high efficiency.

What's inside

Introduction
Chromatographic Similarity
Experimental Methods
Compound List
Discussion on Selectivity

Frequently asked questions

What is the maximum pressure limit for the Agilent Poroshell 120 EC-C18 column?

The maximum pressure limit for the Agilent Poroshell 120 EC-C18 column is 356 bar.

What is the flow rate used in the generic gradient analysis described in the document?

The flow rate used in the generic gradient analysis is 2.0 mL/min.

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