This document introduces a fast method for analyzing environmental phenols by using Agilent Poroshell 120 EC-C18 4.6 × 100 mm, 2.7 µm column, which transfers the method originally developed on a 4.6 × 100 mm, 5-µm column. The gradient and flow rate are scaled while maintaining a constant retention index in order to determine the optimal flow rate for each evaluated column. Simple guidelines for transferring a method are provided. The new separation reduces the analysis time from 20 minutes to less than 3 minutes with a higher peak capacity, and should not require any changes to sample preparation since both columns use 2 µm frits. The method is further scaled to a 50 mm column that produces slightly less resolution than found with the 100 mm column, but with an analysis time of only 2 minutes with an equivalent peak capacity to the original method. While pressure is increased from the original method, it is still below 400 bar and can be easily transferred to any HPLC system.
This document details a method for separating nine phenol compounds originally developed on a 5-um column, transferred to an Agilent Poroshell 120 EC-C18 2.7 um column. The method reduces analysis time from 20 minutes to less than 3 minutes while maintaining peak capacity and not requiring changes to sample preparation. The technique is compatible with standard HPLC systems operating below 400 bar.
| Column Type | Agilent Poroshell 120 EC-C18 |
|---|---|
| Analysis Time | less than 3 minutes |
| Pressure Limit | below 400 bar |
| Flow Rate Range | 0.5 mL/min to 3.5 mL/min |
| Column Length Options | 4.6 x 100 mm and 4.6 x 50 mm |
The new separation reduces the analysis time from 20 minutes to less than 3 minutes.
No, the method should not require any changes to sample preparation since both columns use 2 um frits.
Yes, the pressure is below 400 bar and can be easily transferred to any HPLC system.