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Agilent DNA Polymerase Selection Guide
This document serves as a selection guide for various Agilent DNA polymerases used in PCR applications. It provides a comparative overview of different enzyme types, including standard Taq, high-fidelity proofreading enzymes like PfuUltra and Herculase, and specialized variants designed for difficult targets such as high GC content or dUTP poisoning. The guide details performance metrics such as error rates, reaction speed, and maximum target length for genomic DNA.
Read onlineAgilent Taq2000 DNA Polymerase specifications
| Reaction Volume | 50 µl |
|---|---|
| PfuUltra II Fusion HS Fidelity | 1 error per 2.5 million bp |
| PfuUltra II Fusion HS Speed | 15 sec/kb |
| Herculase II Fusion Fidelity | 1 error per 770,000 bp |
| Paq5000 Fidelity | Equivalent to Taq |
| PfuUltra DNA Polymerase AD Fidelity | 1 error per 2.5 million bp |
| PfuTurbo Cx Hotstart Fidelity | 1 error per 770,000 bp |
| Easy-A PCR Cloning Enzyme Function | Adds 3' -A overhangs |
All Agilent Taq2000 DNA Polymerase documents by type
Questions answered in these manuals
Which polymerase overcomes dUTP poisoning?
ArchaeMaxx is designed to overcome dUTP poisoning, and PfuTurbo Cx Hotstart is the only high-fidelity polymerase that will incorporate dUTP.
Which polymerase is recommended for high GC content targets?
Herculase II Fusion is the first routine, high-fidelity polymerase for difficult targets including high GC content.
What is the reaction volume for the provided PCR protocol?
The protocol specifies a final reaction volume of 50 µl.
From: Agilent Technologies Taq2000 DNA Polymerase Certificate of Analysis
What is the duration of the initial denaturation step?
The initial denaturation at 95°C lasts for 1 minute.
From: Agilent Technologies Taq2000 DNA Polymerase Certificate of Analysis
What is the recommended storage condition for Taq2000 DNA Polymerase?
The product should be stored at -20°C immediately after receipt.
From: Agilent Technologies Taq2000 DNA Polymerase Product Certificate
How is the enzyme activity defined for this polymerase?
One unit is the amount required to incorporate 10 nanomoles of dTTP in 30 minutes at 72°C.
From: Agilent Technologies Taq2000 DNA Polymerase Product Certificate