G4240-62001 HPLC-Chip is the standard chip for one-dimensional separations. It is comprised of a 40 nL enrichment column and a 75 μm x 43mm separation column which is packed with Zorbax 300SB-C18 5 μm material. The HPLC-Chip is made from biocompatible polyimide and the functionality of this chip is equivalent to conventional nanospray LC/MS. ZORBAX 300SB-C18 is a micro particulate column packing designed specifically for reversed-phase HPLC of peptides and proteins. The StableBond packing is made by chemically bonding a sterically protected octadecyl stationary phase to a specially prepared, ultra-high- purity, ZORBAX, porous-silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely covered, sterically protected diisobutyl n-octadecyl stationary phase is chemically stable and gives longer column life. As a result, ZORBAX 300SB-C18 is a stable, reversed-phase packing at low pH that can be used for peptide maps, and separation of synthetic and natural peptides and proteins. It is particularly well suited for use with aggressive mobile phases (e.g., pH < 2, high ionic strength, ion-pair additives, TFA, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This characteristic is particularly important for use in methods that need long-term stability and reproducibility. ZORBAX 300SB-C18 is well suited to applications that utilize weak acidic ion-pair additives as the mobile phase, such as those used for peptide analysis with TFA.
This document describes the Agilent G4240-62001 HPLC-Chip, a standard chip for one-dimensional separations comprised of a 40 nl enrichment column and a 75 ym x 43mm separation column packed with ZORBAX 300SB-C18 5 ym material. The chip is made from biocompatible polyimide and offers functionality equivalent to conventional nanospray LC/MS.
| Enrichment column volume | 40 nl |
|---|---|
| Separation column dimensions | 75 ym x 43mm |
| Packing material | ZORBAX 300SB-C18 5 ym |
| Controlled pore size | 300A |
| Maximum operating pressure | 150 bar (2175 psi) |
| Recommended flow rate | 0.2 - 0.6 ul/min |
The maximum operating pressure for the HPLC-Chip is 150 bar (2175 psi).
Long term storage of silica-based columns should be in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.
Typical flow rates recommended for the HPLC-Chip separation column are 0.2 - 0.6 ul/min.