Agilent ZORBAX RRHD 300SB-C18 1.8 µm is a new reverse phase media for UHPLC analysis of proteins and peptides. By utilizing StableBond technology, it provides a stable 300Å pore-size media under acidic conditions for robust and reproducible protein separations.
The document provides a technical overview of the Agilent ZORBAX RRHD 300SB-C18 1.8 µm column, designed for ultra-high-performance liquid chromatography (UHPLC) of proteins and peptides. It highlights the use of StableBond technology to ensure stability under acidic conditions required for protein analysis.
The text details application examples using short 50 mm columns for intact protein separation at various flow rates and temperatures, as well as longer 100 mm columns for peptide digest analysis. Reproducibility data after multiple runs is also presented.
It is a reverse phase media designed for UHPLC analysis of proteins and peptides.
The column used for intact protein analysis has dimensions of 2.1 x 50 mm.