This article describes the analysis of starches using gel permeation chromatography with viscometry using the Agilent 390-MDS application note. The molecular weight distribution of starches and their properties in various industrial applications such as cooking, textiles, and papermaking are accurately determined using these techniques.
This document details the analysis of starches composed of amylose and amylopectin polymers. The study utilizes Gel Permeation Chromatography (GPC) with Universal Calibration and a viscometer to determine accurate molecular weights independent of column standards. Two different starch samples were analyzed to compare their thickening properties and molecular weight distributions.
| Columns | 3x Agilent PLgel Olexis, 300 x 7.5mm |
|---|---|
| Eluent | DMSO/DMAc (4:1) (0.1% LiBr) |
| Flow Rate | 1.0 mL/min |
| Column Temperature | 60 °C |
| Detector Temperature | 60 °C |
Gel permeation chromatography (GPC) with Universal Calibration, employing a viscometer in combination with a differential refractive index detector.
The samples showed stark differences in molecular weight distributions, with one having a bi-modal distribution, and structural differences indicated by Mark-Houwink plots.