Analysis of Polysaccharides by GPC Viscometry using the Agilent 390-MDS Multi Detector Suite Application Note

About this manual

Polysaccharides are complex polymers where structural differences, such as branching, significantly influence properties like solution viscosity. This document details an analysis of two specific samples: pullulan, which has a linear structure, and dextran, which is highly branched.

The study utilizes Gel Permeation Chromatography (GPC) viscometry employing the Agilent 390-MDS Multi Detector Suite. This system combines a viscometer with a differential refractive index detector to accurately determine molecular weights and investigate solution viscosity via the Universal Calibration approach.

What's inside

• Introduction
• Methods and Materials
• Conditions
• Detector Train
• Results and Discussion
• Conclusion

Frequently asked questions

What polysaccharide samples are analyzed in this application note?

The document describes the analysis of pullulan, which has a linear structure, and dextran, which is highly branched.

What are the dimensions of the columns used for the analysis?

The procedure uses 2x Agilent PL aquagel-OH MIXED-M columns that measure 300 x 7.5 mm.

What is the flow rate for the eluent in this method?

The analysis requires a flow rate of 1.0 mL/min.