Key facts
- Brand
- Agilent
- Model
- Poroshell 300SB-C18
- Category
- Other Electronics
- Documents
- 6
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Poroshell 300SB-C18 High Velocity Chromatography Technical Note
This document introduces Poroshell technology, a superficially porous silica-micrasphere packing designed for high-velocity separations of biomacromolecules. The innovation allows for stable, high-resolution gradient chromatography of proteins, polypeptides, and nucleic acids at speeds up to 400 times faster than conventional high-performance separations without loss of resolution.
The text details the structure of the Poroshell particle, which features a solid silica core with a thin porous silica shell. It highlights the use of StableBond C18 stationary phase, which is stable at low pH and high temperatures, enabling rapid analysis. The document provides examples of protein separations and DNA fragment analysis, demonstrating significant reductions in runtime and back pressure compared to totally porous particles.
Agilent Poroshell 300SB-C18 specifications
| Particle Type | Superficially porous, silica-micrasphere packing |
|---|---|
| Speed Improvement | Up to 400 times faster than conventional separations |
| Shell Pore Size | 300 Angstroms |
| Stationary Phase | StableBond C18 |
| Operating Temperature Limit | 90 degrees Celsius |
| Compatible Equipment | Conventional equipment |
Source: Agilent Poroshell 300SB-C18 High Velocity Chromatography Technical Note
All Agilent Poroshell 300SB-C18 documents by type
Ratings, dimensions and ordering codes, for selection and design work.
How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the main benefit of Poroshell technology?
It enables stable, high-resolution gradient chromatography of biomacromolecules at up to 400-times faster speeds than conventional separations.
From: Agilent Poroshell 300SB-C18 High Velocity Chromatography Technical Note
Can Poroshell columns be used with existing equipment?
Yes, Poroshell packings can be used with conventional equipment without any loss in performance.
From: Agilent Poroshell 300SB-C18 High Velocity Chromatography Technical Note
What is the maximum operating pressure for this column?
The maximum operating pressure is 400 bar or 6000 psi.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What are the pH limits for using this column?
The column should be avoided below pH 0.9 or above pH 8.0.
From: Agilent Technologies POROSHELL 300SB-C18 HPLC Column Datasheet
What is the particle size of the Poroshell 300SB-C18 column?
The column uses a 5 micron particle.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet
At what temperature can the column be used for separations?
The column allows separation at temperatures up to 90°C.
From: Agilent Poroshell 300SB-C18 RP-HPLC Column Product Leaflet