This document describes the use of the USP 23 method to separate water-soluble vitamins using StableBond columns, which provide long-term stability and reliability.
This document presents an application for the analysis of water-soluble vitamins (Niacin, Pyridoxine, Riboflavin, and Thiamine) using USP-recommended chromatographic methods. It addresses the need for reproducible and stable columns in pharmaceutical industry routine analyses.
| Column | ZORBAX SB-C18 (L1 packing) (4.6 x 250 mm) |
|---|---|
| Mobile Phase | 7.2 mM Hexane Sulfonate:MeOH:Acetic Acid (73:27:1) |
| Injection Volume | 20 pL |
| Flow Rate | 1 mL/min |
| Detection | UV(280 nm) |
The procedure specifies a ZORBAX SB-C18 column with dimensions of 4.6 x 250 mm.
The analysis requires an injection volume of 20 pL.