Key facts
- Brand
- Agilent
- Model
- 6540 UHD
- Category
- Other Electronics
- Documents
- 3
- Languages
- EN
- Last added
- 2012-07-05
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Agilent 6540 UHD Q-TOF Technical Overview
This document presents a technical comparison between the Agilent 6540 Ultra High Definition (UHD) Accurate-Mass Q-TOF LC/MS system and the Thermo Scientific LTO Orbitrap XL. It focuses on the performance of the Agilent system when used at high data acquisition rates required for fast Ultra High Performance Liquid Chromatography (UHPLC) analyses.
The study demonstrates that the Agilent 6540 UHD Q-TOF maintains high resolution even at very high acquisition rates, whereas the Thermo LTQ Orbitrap XL resolution drops precipitously as acquisition speeds increase. The document concludes that the Agilent system delivers up to ten-fold better resolving power than the Orbitrap XL for this specific application, while maintaining sensitivity, dynamic range, mass accuracy, and isotopic fidelity.
Agilent 6540 UHD specifications
| In-spectrum dynamic range (Agilent 6540 UHD O-TOF) | 50,000 to 100,000 |
|---|---|
| In-spectrum dynamic range (Thermo LTO Orbitrap XL) | 800 |
| Resolution mode for comparison | 15,000 |
| Acquisition rate for comparison | 3.3 spectra/sec |
All Agilent 6540 UHD documents by type
Questions answered in these manuals
What is the minimum number of data points a chromatographer would accept for a peptide peak?
The minimum acceptable number of data points across a peak is 10 to 12.
What resolution does the Thermo LTO Orbitrap XL achieve at an acquisition rate of 5 spectra/second?
At 5 spectra/second, the Thermo LTO Orbitrap XL resolution drops below 10,000 over the examined mass range.
What isotope ratio error level does the Agilent 6540 UHD O-TOF demonstrate?
The system shows a consistently low relative isotope abundance (RIA) error of about 5%.
How does the Agilent MFG software use data to generate molecular formulas?
The software considers the accurate mass of the parent ion, isotope abundance distribution, and mass spacing between isotope peaks.
What is the in-spectrum dynamic range of the Agilent 6540 UHD Q-TOF?
The Agilent 6540 UHD Q-TOF demonstrates an in-spectrum dynamic range of 50,000 to 100,000.
What is the in-spectrum dynamic range of the Agilent 6540 UHD Q-TOF LC/MS system?
The system demonstrated an in-spectrum dynamic range of 50,000 to 100,000.