Agilent ZORBAX SB-C18 Reversed-Phase Separation Application Note

This document introduces a method for separating explosives from soil extracts using an Agilent ZORBAX SB-C18 column. The method uses methanol-water (50:50) as the mobile phase and a detection wavelength of 230 nm.


Agilent ZORBAX SB-C18 Reversed-Phase Separation Application Note - cover page
Brand
Agilent
Category
Other Electronics
Document type
Product Leaflet
Language
English
Pages
2
File format
PDF
File size
208 KB
Published
02 July, 2012
Updated
30 September, 2023
MD5 checksum
FFB74B6EC56FB8DC67869307528FBCB1

About this manual

This document presents a chromatographic method for the reversed-phase separation of 15 different explosives found in soil extracts. The method utilizes a ZORBAX SB-C18 column with a methanol and water mobile phase.

What's inside

  • Application
  • Environmental
  • Reversed-Phase Separation
  • Highlights
  • Conditions

Specifications

ColumnZORBAX SB-C18 (3 x 250 mm)
Column Part Number880975-302
Mobile PhaseMethanol:Water (50:50) (v/v)
Injection Volume10pI
Flow Rate0.3 mL/min
DetectionUV (230 nm)

Frequently asked questions

What column dimensions are required for this separation?

The procedure uses a ZORBAX SB-C18 column with dimensions of 3 x 250 mm.

What is the mobile phase composition for this analysis?

The separation uses a mobile phase consisting of Methanol and Water in a 50:50 ratio by volume.

What detection wavelength is used for this method?

The system detects the analytes using UV light at a wavelength of 230 nm.

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