This document describes the performance comparison of the non-endcapped Agilent ZORBAX SB-C18 column and its doubly endcapped counterpart in the separation of fat-soluble vitamins.
This document presents an application study on the separation of fat-soluble vitamins, specifically focusing on the difficult separation of vitamins D2 and D3. It compares a non-endcapped Agilent ZORBAX SB-C18 column with its doubly endcapped counterpart, the ZORBAX Eclipse XDB-C18 column. Both columns are based on type A silica, but endcapping is performed on the latter to minimize secondary interactions of polar sample components and allow for higher pH operation.
These columns are used for the separation of fat-soluble vitamins, specifically to resolve vitamins D2 and D3.
Both the non-endcapped ZORBAX SB-C18 and the endcapped Eclipse XDB-C18 columns have dimensions of 4.6 x 150.
The flow rate specified for the analysis is 1.0 mL / min.