This document introduces a high-efficiency and high-speed separation method for natural anthocyanins. The method uses ZORBAX StableBond SB-C18 columns and phosphoric acid mobile phase, which can achieve high-efficiency separation of more than 25 anthocyanins.
This document details the application of Agilent ZORBAX StableBond SB-C18 columns for the qualitative and quantitative analysis of anthocyanins, which are natural pigments found in fruits like blueberries. The text highlights that traditional low-pH mobile phases containing formic acid can cause column degradation; instead, this method uses phosphoric acid to ensure long-term stability and superior separation of over 25 different anthocyanins.
Separating anthocyanins helps distinguish between different blueberry cultivars, determine their quality, and analyze their use as antioxidants or anticancer agents.
The mobile phase consists of 3% phosphoric acid and 100% methanol.
The columns used have dimensions of 4.6 x 250 mm, 4.6 x 150 mm, and 4.6 x 75 mm.