This manual describes the use of 1.8-µm ZORBAX SB C18 Rapid Resolution HT columns with the Agilent 1100 Series HPLC system for ultra-fast liquid chromatography.
This document describes the recommended system configuration to achieve very fast analysis and cycle times using ZORBAX 1.8-um Rapid Resolution High Throughput (RRHT) columns on the Agilent 1100 Series liquid chromatograph. It details how to reduce analysis time to seconds without compromising resolution or chromatographic performance.
| Analysis time | 29 seconds |
|---|---|
| Peak width | 0.5 seconds |
| Backpressure | 300 bar |
| Column particle size | 1.8 um |
| Delay volume | 300 pL |
| Injection speed | < 30 seconds |
| Flow cell volume | 500-nL |
The minimum configuration consists of the Agilent 1100 Series binary pump, well-plate autosampler, thermostatted column compartment, and ZORBAX 1.8-um RRHT columns.
Overlapped injection allows the preparation of the next injection while the current injection is being completed, increasing sample throughput.