This document describes the method of using the Varian Star 9080 Amperometric Electrochemical Detector for pulsed amperometric detection of disaccharides. The document provides detailed information on the instrument, column, flow rate, mobile phase, sample, temperature, and flow cell parameters used.
The document details an analytical method for detecting L C disaccharides, specifically alpha-lactose and sucrose, using a Varian Star 9080 Amperometric Electrochemical Detector. The analysis utilizes a Dionex CarboPac PA1 column with a flow rate of 1.0 mL/min.
The detector is used for the pulsed amperometric detection of alpha-lactose, sucrose, and maltose.
The procedure specifies a flowrate of 1.0 mL/min.
The analysis is performed at a temperature of 30°C.