Application note: Analysis of amino acid neurotransmitters with Star 9080 EC detector
This document provides an application note for analyzing amino acid neurotransmitters using the Varian Star 9080 Amperometric Electrochemical Detector. The method involves OPA-sulphite derivatisation of samples, followed by standard separation and detection.
The text details a specific experimental setup including column dimensions (C18, 250 x 4.6mm), flow rate (0.70 mL/min), mobile phase composition, injection volume (20 uL), and detector settings such as the glassy carbon working electrode and applied potential of 850 mV.
The amount injected is 20 pmol (1 pmol/L) for each compound.
Peaks correspond to serine, glycine, taurine, glutamate, arginine, alanine, OPA reagent, and GABA.