The document introduces the application of Star 9080 Amperometric Electrochemical Detector . The detector is used to analyze the standards of 30 nmol/L noradrenaline (1), adrenaline (2) and dopamine (3).
This document details the analysis of catecholamines (noradrenaline, adrenaline, and dopamine) using the Varian Star 9080 Amperometric Electrochemical Detector. It specifies the standard separation conditions, including the use of an ODS column, a mobile phase containing phosphoric acid, citric acid, EDTA, and octane sulphonic acid, and a flow rate of 1.0 mL/min.
| Detector Type | Amperometric Electrochemical Detector |
|---|---|
| Flow Rate | 1.0 mL/min |
| Column Dimensions | 150 x 4.6 mm |
| Injection Volume | 20 uL |
| Flowcell Electrode | 2.74 mm Glassy Carbon |
| Reference Electrode | Ag/AgCl filled with saturated KCI/AgCl |
| E-cell Potential | 800 mV |
| I-cell Current | 3 nA |
A typical S/N ratio for norepinephrine measured with a VT-03 glassy carbon flowcell is 53000.
The concentration detection limit based on three times the noise is 56 pmol/L.
The minimum detectable amount is 1.12 fmol.