This document introduces the application of using Varian Star 9080 EC Detector to analyze phenol in water
This document provides an application note for analyzing a standard mixture of priority pollutants in water using the Varian Star 9080 Amperometric Electrochemical Detector. The analysis targets compounds such as 2,4-dinitrophenol, phenol, and various chlorophenols listed by the US EPA.
The procedure utilizes an ODS column (100 x 4.6 mm) with a flow rate of 1.5 mL/min. The mobile phase consists of 50 mM HAc/NaAc at pH 4.0 with 35% acetonitrile. The detector operates with a flowcell of 2.74 mm Glassy Carbon working electrode and an E-cell voltage of 1200 mV.
| Column | ODS, 100 x 4.6 mm |
|---|---|
| Flowrate | 1.5 mL/min |
| Mobile phase | 50 mM HAc/NaAc, pH 4.0, 35% acetonitrile |
| Sample concentration | 100 - 1000 nM phenols |
| Injection volume | 20 uL |
| Temperature | 30°C |
| Flowcell | 2.74 mm Glassy Carbon working electrode |
| E-cell voltage | 1200 mV (vs. Ag/AgCl) |
The mixture contains 2,4-dinitrophenol, phenol, 4-nitrophenol, 2-methyl-4,6-dinitrophenol, 2-chlorophenol, 2-nitrophenol, 2,4-dimethylphenol, 4-chloro-3-methylphenol, 2,4-dichlorophenol, and 2,4,6-trichlorophenol.
The recommended injection volume is 20 uL for samples containing 100 - 1000 nM phenols.
The column temperature is set to 30°C.