This document describes the SAMHSA-compliant LC/MS/MS analysis of benzoylecgonine in urine using Agilent Bond Elut Plexa PCX and Agilent Poroshell 120. The method's linearity, limit of detection (LOD), accuracy, and precision are demonstrated, as well as measurement of matrix effects, extraction recovery, and overall process efficiency.
This document presents a method for the analysis of benzoylecgonine that meets the most recent SAMHSA guidelines. The method utilizes Agilent Bond Elut Plexa PCX mixed-mode polymeric SPE sorbent and Agilent Poroshell 120 EC-C18 superficially porous LC columns. It demonstrates linearity, limit of detection, accuracy, and precision, as well as measurement of matrix effects and extraction recovery.
| SPE Cartridge | Agilent Bond Elut Plexa PCX, 30 mg, 3 mL |
|---|---|
| MS System | Agilent 6460 Triple Quadrupole LC/MS system |
| Flow rate | 0.8 mL/min |
| Max pump pressure | 400 bar |
| Extraction recovery | 86% |
| Matrix effect | 99% |
| Accuracy | 102% |
| Precision (CV) | 0.7% |
The SAMHSA-established confirmation cutoff concentration for benzoylecgonine is 100 ng/mL.
The LOD at 10% of the cutoff is 10 ng/mL.
The extraction recovery for benzoylecgonine is 86%.