This article presents a SAMHSA-compliant LC/MS/MS analysis method for the detection of 6-acetylmorphine in urine. The method utilizes Agilent products such as Agilent Bond Elut Plexa PCX and Agilent Poroshell 120, and demonstrates simplification, linearity, limit of detection (LOD), accuracy, and precision.
The document presents a simplified liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the confirmation of 6-acetylmorphine in urine, meeting US Substance Abuse and Mental Health Services Administration (SAMHSA) guidelines effective October 2010. The method utilizes Agilent Bond Elut Plexa PCX mixed-mode polymeric SPE sorbent to minimize ion suppression and maximize sensitivity.
The analytical procedure employs an Agilent Poroshell 120 EC-C18 superficially porous column for high-capacity separation and an Agilent 6460 Triple Quadrupole LC/MS system with AJST electrospray source. The method demonstrates excellent linearity, limit of detection (LOD), accuracy, precision, and extraction recovery suitable for forensic toxicology applications.
The updated SAMHSA confirmation cutoff concentration for 6-AM is 10 ng/mL.
A LOD at 10% of the cutoff would be 1 ng/mL.
The extraction procedure yields an extraction recovery of 83%.