Agilent Technologies, Inc. has released a new SAMHSA method for the LC/MS/MS analysis of phencyclidine in urine. The method is simple and easy to use, does not require derivatization, and exhibits good performance in terms of a wide range, high accuracy, high recovery, and overall process efficiency.
This document presents a method for the analysis of phencyclidine (PCP) in urine that meets the guidelines set by the US Substance Abuse and Mental Health Services Administration (SAMHSA). The method utilizes Agilent Bond Elut Plexa PCX mixed-mode polymeric SPE sorbent and Agilent Poroshell 120 EC-C18 columns to achieve high recovery and sensitivity. It details the use of an Agilent 6460 Triple Quadrupole LC/MS system with AJST enhanced electrospray source for detection.
| SPE Cartridge Capacity | 30 mg |
|---|---|
| Column Dimensions | 3 x 50 mm |
| Column Particle Size | 2.7 ym |
| Flow Rate | 0.8 mL/min |
| Injection Volume | 2 yL |
| Capillary Voltage | 3,000 V |
| Drying Gas Temperature | 350 °C |
| Sheath Gas Temperature | 400°C |
| Calibration Range | 2.5 to 1000 ng/mL |
| Linearity (R2) | > 0.999 |
The new SAMHSA confirmation cutoff concentration for phencyclidine is 25 ng/mL.
The method demonstrates a LOD at 10% of the cutoff, which is 2.5 ng/mL.
In frozen urine samples, phencyclidine is preserved for a year.