This document presents a method for SAMHSA-compliant LC/MS/MS analysis of opiates (morphine and codeine) in urine using Agilent Bond Elut Plexa PCX and Agilent Poroshell 120. The method meets the latest SAMHSA guidelines and demonstrates linearity, limit of detection (LOD), accuracy and precision, as well as measurement of matrix effects, extraction recovery, and overall process efficiency. The document also introduces the Agilent products used and their features.
The document presents a simplified liquid chromatography-tandem mass spectrometry (LC/MS/MS) method designed to meet the guidelines of the US Substance Abuse and Mental Health Services Administration (SAMHSA). The method focuses on the confirmation of opiates, specifically morphine and codeine, in urine samples without requiring derivatization steps. It utilizes Agilent Bond Elut Plexa PCX mixed-mode polymeric solid-phase extraction (SPE) cartridges for sample preparation.
The established confirmation cutoff concentration for morphine and codeine is 2,000 ng/mL.
The sample should be incubated in the heating block at 95 +5 °C for 90 minutes.