Key facts
- Brand
- Agilent
- Model
- 971-FP
- Industry
- Industrial
- Documents
- 6
- Languages
- EN
- Last added
- 2012-07-05
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Agilent 971-FP Flash Purification System IntelliFlash Guide Me Scale-Up Feature Guide
The document describes the 'Scale Up' feature within the IntelliFlash software for the Agilent 971-FP Flash Purification System. This feature allows users to transfer method details between Agilent SuperFlash columns of different sizes while maintaining compound purity and yield, without requiring manual calculations of column dimensions or flow rates.
The guide details the mathematical principles behind the scaling process, specifically the equations used to calculate flow rate for the same linear velocity and gradient duration for different column lengths. It provides instructions on how to use the software interface to select historical runs or specify parameters to automatically generate new run conditions for scaled-up separations.
Agilent 971-FP specifications
| Column Length | Longest column in the SuperFlash column product range |
|---|---|
| Media Type | 35 µm silica |
| Flow Rate | 40 mL/min |
| Purification Range | 1.6 g to 16 g |
| Aspect Ratio | 16.2:1 |
Source: Agilent 971-FP Flash Purification System Technical Overview
All Agilent 971-FP documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
How to use the product for one specific task, with worked examples.
Disassembly, diagnostics and part numbers, for repair.
Questions answered in these manuals
How does dual detection prevent sample loss?
It allows the isolation of non-UV active compounds that would otherwise remain undetected by a UV detector alone.
From: Varian 971-FP Flash Chromatography Detection Method Application Note
What instruments are used in this demonstration?
A Varian 385-LC ELS detector coupled with a Varian 971-FP flash instrument.
From: Varian 971-FP Flash Chromatography Detection Method Application Note
Can the SF25-160 g Si 35 column be run at 40 mL/min?
Yes, it can be run at 40 mL/min without exceeding the maximum operating pressure of the Agilent 971-FP instrument.
From: Agilent 971-FP Flash Purification System Technical Overview
What is the purification range for the SF25-160 g Si 35 column?
It is designed for the purification of between 1.6 g and 16 g of a complex mix.
From: Agilent 971-FP Flash Purification System Technical Overview
Does the software require manual calculation of column dimensions for scale-up?
No, the software automatically supplies the necessary calculations for flow rate and duration.
From: Agilent 971-FP Flash Purification System IntelliFlash Guide Me Scale-Up Feature Guide
What parameters are critical for successful purification scale-up?
The flow rate used to run the column and the number of column volumes needed for the gradient.
From: Agilent 971-FP Flash Purification System IntelliFlash Guide Me Scale-Up Feature Guide