This document introduces the use of the IntelliFlash software “Guide Me” feature for automatic scale-up of purification.
The document describes the 'Scale Up' feature within the IntelliFlash software for the Agilent 971-FP Flash Purification System. This feature allows users to transfer method details between Agilent SuperFlash columns of different sizes while maintaining compound purity and yield, without requiring manual calculations of column dimensions or flow rates.
The guide details the mathematical principles behind the scaling process, specifically the equations used to calculate flow rate for the same linear velocity and gradient duration for different column lengths. It provides instructions on how to use the software interface to select historical runs or specify parameters to automatically generate new run conditions for scaled-up separations.
| Compatible Instrument | Agilent 971-FP Flash Purification System |
|---|---|
| Compatible Columns | Agilent SuperFlash columns |
| Publication Date | April 1, 2011 |
No, the software automatically supplies the necessary calculations for flow rate and duration.
The flow rate used to run the column and the number of column volumes needed for the gradient.