This document demonstrates the benefits of using an evaporative light scattering detector (ELSD) coupled to the Agilent 971-FP Flash instrument for the analysis of a sample containing a mixture of UV and non-UV-active compounds. The ELSD can detect compounds without UV chromophores, preventing the loss of these compounds that would go undetected by UV and be discarded.
This document describes a method for flash purification of compounds that lack UV chromophores using an Evaporative Light Scattering Detector (ELSD). It highlights the benefits of coupling an Agilent 385-ELSD with an Agilent 971-FP Flash instrument to prevent the loss of sample components that are undetected by UV alone.
The note details a specific experiment using a mixture of B-carotene, tocopherols, and aminophenols. It explains how the software allows direct control of the ELSD settings and how fraction collection can be triggered by both UV and ELSD responses to ensure all components are collected.
| Column | SF15-12g Si 35 |
|---|---|
| Eluent A | Hexane |
| Eluent B | Ethyl acetate |
| Flow Rate | 18 mL/min |
| Injection Volume | 0.5mL |
| Temperature | Ambient |
| UV Detection Wavelength | 254 nm |
| ELSD Nebulizer Temperature | 15°C |
| ELSD Evaporation Temperature | 30 °C |
| ELSD Gas Flow | 1.2 SLM |
It prevents the loss of sample components that remain undetected by UV and are therefore not isolated by the fraction collector.
The software permits direct control of the ELSD via an on-screen user interface, allowing the operator to change detection settings as required.
Fraction collection is triggered by both the UV and ELSD responses to ensure all components are collected.