The document describes the features and characteristics of the Agilent 971-FP Flash Purification System and its application in discovery applications. The system allows purification of complex samples by running the longest Agilent SuperFlash column at a flow rate of 40 mL/min without exceeding the maximum operating pressure. Agilent offers a complete flash purification solution including the 971-FP instrument, IntelliFlash software, and SuperFlash columns for drug discovery applications. These applications typically require purification of milligram to several gram quantities of a synthetic compound from a synthesis mix. To achieve high efficiency for related compounds, smaller particle media and longer column lengths are used. The SuperFlash SF25-160 g Si 35 column is a normal phase column packed with high efficiency 35 µm silica. It has an aspect ratio of 16.2:1 and is designed for purifying complex mixes in the range of 1.6 g to 16 g. Investigations of the operating pressure of this column determined the maximum pressure reached during purification using the IntelliFlash software at the default flow rate for this column id and a gradient from 0 to 100% ethyl acetate.
This document provides a technical overview of the Agilent 971-FP Flash Purification System, specifically addressing the capability of the longest SuperFlash column (SF25-160 g Si 35) for drug discovery applications. It details how the system can purify complex samples ranging from 1.6 g to 16 g using high-efficiency 35 µm silica media.
| Column Length | Longest column in the SuperFlash column product range |
|---|---|
| Media Type | 35 µm silica |
| Flow Rate | 40 mL/min |
| Purification Range | 1.6 g to 16 g |
| Aspect Ratio | 16.2:1 |
Yes, it can be run at 40 mL/min without exceeding the maximum operating pressure of the Agilent 971-FP instrument.
It is designed for the purification of between 1.6 g and 16 g of a complex mix.