This document describes the quantitation of a dinitro flavonoid (DNF) in rat plasma using the Varian 1200L LC/MS/MS system. Flavonoids are compounds that make up the pigments in fruits, vegetables, and herbs. There have been reports of flavonoids having multiple beneficial therapeutic effects for a large number of serious illnesses, such as rheumatoid arthritis, cancer, cataracts, anxiety, and depression. The objective of this work was to carry out a pharmacokinetic evaluation to determine a profile with the formulation being tested at increasing doses where there is pharmacological effect. A Varian 1200L LC/MS/MS system was used for the quantitation of plasma levels of a novel dinitro-substituted flavone (DNF) in samples from a pharmacokinetic study following oral administration of the drug. Flavone was used as the internal standard. Structures are shown in Figure 1.
This document details a pharmacokinetic evaluation method for determining the profile of a novel dinitro-substituted flavone (DNF) in rat plasma. The study utilized oral administration and analyzed samples to observe concentration changes over time, noting phenomena such as entero-hepatic recirculation.
The analysis was performed using an Agilent 1200L LC/MS/MS system equipped with an ESI source. The method involves extracting DNF from plasma using hexane:chloroform and analyzing it via Selected Reaction Monitoring (SRM). Specific HPLC conditions, MS parameters, and scan settings for the analyte and internal standard are provided.
A Varian (now Agilent) 1200L LC/MS/MS system equipped with an ESI source.
Flavone was used as the internal standard.
The calibration curve range is from 0.10 ng/mL to 5.00 ng/mL of rat plasma.