The document demonstrates the improved liquid chromatography analysis of complex biological fluids using fine particle columns by comparing two columns of different lengths and particle sizes on the analysis of a tryptic digest of human serum albumin (HSA). The columns provide better resolution and comparable sensitivity to 150-mm columns, but in one-sixth the time.
The document compares the performance of standard 5-µm particle ZORBAX SB-C18 columns with newer 1.8-µm particle ZORBAX Rapid Resolution HT SB-C18 columns for analyzing human serum albumin (HSA) tryptic digests. The analysis shows that using shorter, high-throughput columns significantly improves resolution and reduces analysis time.
| Particle Size | 1.8 µm |
|---|---|
| Pore Size | 80 Å |
| Temperature | 50°C |
| Injection Volume | 8 µL |
The part number for the 2.1 x 50 mm, 1.8 µm column is 822700-902.
The analysis time is reduced by one-sixth, or 500% faster.