Agilent ZORBAX SB-C8 is a stable, reversed-phase packing that can be used for basic, neutral, or acidic samples. It is particularly well-suited for use with aggressive mobile phases (for example, pH < 2, high ionic strength, ion-pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility.
This document describes the Agilent ZORBAX Rapid Resolution High Definition (RRHD) SB-C8 threaded column, specifically engineered for use with ultra-high performance liquid chromatographs (UHPLCs). The column utilizes a StableBond C8 stationary phase packed on high-purity ZORBAX porous silica microspheres to provide high-speed and high-resolution reversed-phase HPLC.
| Maximum Operating Pressure | 1200 bar (17,000 psi) |
|---|---|
| Maximum Operating Temperature | 80 °C at low pH only |
| Particle Size | 1.8 µm |
| Pore Size | 80 Å |
These RRHD columns support operation up to 1200 bar (17,000 psi).
Avoid using these columns below pH 1.0 or above pH 8.0.
Store in a pure organic solvent, preferably an aprotic liquid such as 100% acetonitrile.