Polaris 5µ C18-A column can be used to separate Cysteine underivatized, the column length is 250mm, the inner diameter is 4.6mm. The mobile phase is 50mM NH4H2PO4 + 1 mM sodium heptane sulfonate, pH is 2.5, and the flow rate is 1.0 ml/min. The detection wavelength is 210 nm.
This document is an application note from Agilent Technologies regarding the analysis of L-cysteine. It specifies the use of a Polaris 5u C18-A column with dimensions 250 x 4.6mm and part number A2000250X046.
The recommended chromatographic conditions include an eluent A of 50 mM NH4H2PO4 + 1 mM sodium heptane sulfonate at pH 2.5, and an eluent B of MeCN. The gradient starts with a 95:5 ratio of A:B, a flow rate of 1.0 ml/min, and UV detection at 210 nm.
| Column | Polaris 5u C18-A, 250 x 4.6mm |
|---|---|
| Part Number | A2000250X046 |
| Eluent A | 50 mM NH4H2PO4 + 1 mM sodium heptane sulfonate, pH 2.5 |
| Flow Rate | 1.0 ml/min |
| Detection | UV @ 210 nm |
The application uses a Polaris 5u C18-A column with dimensions 250 x 4.6mm.
Eluent A is 50 mM NH4H2PO4 + 1 mM sodium heptane sulfonate at pH 2.5, and Eluent B is MeCN.