The document is about application note, it introduces the application method and the instruments and reagents used.
This document is an application note from Agilent Technologies regarding the analysis of antidiabetics. It references the acquisition of Varian, Inc. by Agilent and directs users to the company website for further information.
The note specifies the use of the Polaris 5u C18-A column (Part nr. A2000150X046) with dimensions of 150 x 4.6mm. The recommended eluent is a mixture of MeCN and 75 mM (NH4)3PO4 at pH 4.4 in a 70:30 ratio, with a flow rate of 21.0 ml/min and UV detection at 254nm. The chromatogram identifies glyburide as a peak.
| Column | Polaris 5u C18-A, 150 x 4.6mm |
|---|---|
| Part Number | A2000150X046 |
| Eluent | MeCN : 75 mM (NH4)3PO4, pH 4.4, 70 : 30 |
| Flow Rate | 21.0 ml/min |
| Detection | UV @ 254nm |
The Polaris 5u C18-A column is used.
The part number is A2000150X046.
The flow rate is 21.0 ml/min.