This document provides instructions for using BL21(DE3) Competent Cells, BL21(DE3)pLysS Competent Cells, and BL21 Competent Cells, including storage conditions, ordering information, and technical services.
This document provides instructions for the use of three specific strains of Escherichia coli competent cells: BL21(DE3), BL21(DE3)pLysS, and BL21. These strains are designed for high-level protein expression using T7 promoter-driven vectors such as pCAL and pET systems.
| Storage Temperature | -80°C |
|---|---|
| Recommended Heat Pulse Duration | 45-50 seconds |
The competent cells must be stored at -80°C immediately upon arrival and should not be stored in liquid nitrogen.
Optimal results are achieved when reactions are heat-pulsed for 45-50 seconds.
It is important to use exactly 100 ul of competent cells for each transformation.