Key facts
- Brand
- Agilent
- Model
- 2-Step
- Documents
- 2
- Languages
- EN
- Last added
- 2012-06-06
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Agilent SideStep II SYBR Green QRT-PCR Master Mix 2-Step Instruction Manual
The document serves as an instruction manual for the Agilent SideStep II SYBR Green QRT-PCR Master Mix, 2-Step (Catalog #400909). It details the materials provided in the kit, including reagents for cell lysis, cDNA synthesis, and QPCR amplification. The guide outlines storage conditions and lists additional materials required for use.
Read onlineAgilent 2-Step specifications
| Reaction Volume (cDNA synthesis) | 20-μl reaction volume |
|---|---|
| QPCR Reactions Capacity | 400, 25-μl reactions |
| Lysate Samples Capacity | 100 cell lysate samples |
| Storage Temperature (Kit) | -20°C upon receipt |
| Lysate Stability | at least 20 months |
Source: Agilent SideStep II SYBR Green QRT-PCR Master Mix 2-Step Instruction Manual
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Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
Is this product for diagnostic procedures?
No, the product is for research use only and not for use in diagnostic procedures.
From: Agilent SideStep II SYBR Green QRT-PCR Master Mix 2-Step Instruction Manual
How long are SideStep lysates stable after thawing?
SideStep lysates are suitable for QRT-PCR analysis for at least 20 months when stored at –80°C.
From: Agilent SideStep II SYBR Green QRT-PCR Master Mix 2-Step Instruction Manual
What additional equipment is required for this kit?
A spectrofluorometric thermal cycler and nuclease-free PCR-grade water are required.
What is the storage condition for the master mix after thawing?
Store the 2x master mix at 4°C after thawing.