Key facts
- Brand
- Agilent
- Model
- Protein 230
- Documents
- 4
- Languages
- EN
- Last added
- 2012-07-05
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Agilent Protein 230 Kit Guide
The document serves as a user manual for the Agilent Protein 230 Kit, detailing the necessary equipment and supplies required for protein analysis. It outlines the setup procedures for both the Chip Priming Station and the Bioanalyzer instrument before beginning the assay.
The guide provides step-by-step protocols for preparing reagents such as Gel-Dye Mix and Denaturing Solution, loading samples onto Protein Chips, running assays, and interpreting results via the 2100 Expert software. It also includes technical specifications regarding sizing ranges, sensitivity, and compatible buffers.
Agilent Protein 230 specifications
| Sizing range | 14 to 230 kDa |
|---|---|
| Lower marker size | 4.5 kDa |
| Ladder purity | >98 % |
| Sensitivity (CAII in PBS) | 6 ng/pL |
Source: Agilent New Series II Protein 230 assay for the Agilent 2100 bioanalyzer Manual
All Agilent Protein 230 documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the sizing range of the Protein 230 assay?
The Protein 230 kit allows for confident analysis of proteins ranging from 14 to 230 kDa.
From: Agilent New Series II Protein 230 assay for the Agilent 2100 bioanalyzer Manual
How long is the lifetime of the Protein 230 reagents when stored appropriately?
The lifetime of the Protein 230 reagents was extended to a minimum of four months if stored appropriately.
From: Agilent New Series II Protein 230 assay for the Agilent 2100 bioanalyzer Manual
What is the basic principle for protein sizing?
Protein size determination is calculated via a standard curve from the ladder, which converts measured migration times of unknown proteins to their sizes.
From: Agilent Protein Sizing Quantitation Technical Overview for Protein 80/230 Kits and 2100 Bioanalyzer
How is relative protein concentration calculated?
Relative concentration is calculated by comparing peak areas against an internal upper marker added in a defined ratio to each sample.
From: Agilent Protein Sizing Quantitation Technical Overview for Protein 80/230 Kits and 2100 Bioanalyzer
What is the sizing range for the Protein 230 Assay?
The assay covers a sizing range of 14-230 kDa.
How many samples can be analyzed per chip?
Up to 10 samples can be loaded onto a single chip.