Key facts
- Brand
- Agilent
- Model
- 1-Step
- Documents
- 3
- Languages
- EN
- Last added
- 2012-06-06
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Agilent SideStep II QRT-PCR Master Mix Kit 1-Step Instruction Manual
The document details the use of the Agilent SideStep II QRT-PCR Master Mix Kit, which combines two modules to analyze gene expression in mammalian cells. The first module is a Cell Lysis Analysis Kit that allows for rapid preparation of lysates by skipping conventional nucleic acid purification steps, utilizing a buffer that stabilizes nucleic acids and inactivates cellular enzymes.
The second module is the Brilliant II QRT-PCR Master Mix Kit, 1-Step, used for probe-based detection protocols. The manual outlines specific procedures for preparing SideStep cell lysates, performing one-step QRT-PCR analysis on these lysates, normalizing variations in nucleic acid concentration using genomic DNA targets, and troubleshooting common issues.
Agilent 1-Step specifications
| Reactions supported by normalization primers (Set 1) | fifty, 25-μl QPCR reactions |
|---|---|
| Reactions supported by QRT-PCR reagents | four hundred, 25-μl reactions |
| Storage temperature (upon receipt) | -20°C |
| Stability of SideStep lysates | at least 20 months when stored at –80°C |
| Guaranteed activity after thawing | 6 months |
Source: Agilent SideStep II QRT-PCR Master Mix Kit 1-Step Instruction Manual
All Agilent 1-Step documents by type
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in these manuals
What is the purpose of the QPCR Normalization Primers?
They amplify distinct, single-copy regions of noncoding human genomic DNA to quantitate genomic DNA content for normalizing gene expression data or determining cell concentration.
From: Agilent SideStep II QRT-PCR Master Mix Kit 1-Step Instruction Manual
How long are SideStep lysates stable after preparation?
They are suitable for analysis for at least 20 months when stored at –80°C.
From: Agilent SideStep II QRT-PCR Master Mix Kit 1-Step Instruction Manual
How should the master mix be stored after thawing?
Store at 4°C. Once thawed, full activity is guaranteed for 6 months.
From: Agilent Technologies Brilliant II SYBR Green QRT-PCR Master Mix with ROX 1-Step INSTRUCTION MANUAL
What is the maximum recommended length for amplicons when using this kit?
The kit is ideal for amplifying targets that are up to 300 base pairs long.
From: Agilent Technologies Brilliant II SYBR Green QRT-PCR Master Mix with ROX 1-Step INSTRUCTION MANUAL
What additional materials are required?
A spectrofluorometric thermal cycler and nuclease-free PCR-grade water.
From: Agilent Technologies BrilliantIISYBR GreenQRT-PCR Master Mix Kit 1-Step INSTRUCTION MANUAL
What reaction temperature does the reverse transcriptase perform optimally at?
The reverse transcriptase performs optimally at a reaction temperature of 50°C.
From: Agilent Technologies BrilliantIISYBR GreenQRT-PCR Master Mix Kit 1-Step INSTRUCTION MANUAL