Key facts
- Brand
- Agilent
- Model
- HT-2
- Industry
- Industrial
- Documents
- 2
- Languages
- EN
- Last added
- 2012-07-05
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Agilent 5100 Protein 200 HT-2 Assay Technical Note
The Protein 200 HT-2 assay is run on the Agilent 5100 Automated Lab-on-a-Chip platform (5100 ALP) to size and quantitate proteins ranging from 15 to 220 kDa. Analysis is performed under denaturing conditions, with or without a reducing agent, in an automated high-throughput mode using standard 96-well plates.
The assay utilizes the Protein 200 HT-2 chip and reagent kit. Proteins are separated sequentially through a sieving polymer in the chip's separation channel and detected by laser-induced fluorescence. The 5100 expert HT software generates a standard curve using a protein ladder to calculate molecular weights and concentrations based on migration time and fluorescence intensity.
Agilent HT-2 specifications
| Sizing range | 15 to 220 kDa |
|---|---|
| Chip part number | G3011-87300 |
| Reagent kit part number | G3011-68750 |
| Instrument part number | G3002A |
| Sample volume | 4 pL |
| Denaturation temperature | 95 °C |
| Ladder protein sizes | 15, 28, 46, 63, 95, 150 kDa |
| Upper marker size | 240 kDa |
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How to use the product for one specific task, with worked examples.
Questions answered in these manuals
What is the sizing range of the Protein 200 HT-2 assay?
The assay allows for sizing of proteins ranging from 15 to 220 kDa.
Can the Protein 200 HT-2 assay be performed without a reducing agent?
Yes, analysis is performed under denaturing conditions in the absence or presence of a reducing agent.
What is the linear dynamic range for non-reduced antibody 1?
A good linearity (R² ≥ 0.98) is achieved up to 1000 pg/mL.
From: Agilent 5100 Protein 200 HT-2 Assay Antibody Analysis Application Note
What is the linear dynamic range for reduced antibody 2?
A good linear dynamic range is achieved up to 2000 pg/mL.
From: Agilent 5100 Protein 200 HT-2 Assay Antibody Analysis Application Note