Agilent 500-MS Ion Trap LC/MS Application Note for Ethomeen T/20H Characterization

About this manual

The document details a method to analyze the multi-compound, polymeric emulsifier Ethomeen T/20H. The analysis utilizes Liquid Chromatography-Mass Spectrometry (LC/MS) with TurboDDS software capabilities on an Agilent 500-MS Ion Trap Mass Spectrometer equipped with an ESI source.

The method involves separating the components of the emulsifier using HPLC conditions specified in the text, followed by data-dependent scanning to identify precursor ions and perform MS^n analyses. The results demonstrate that mass spectral peaks spaced 44 m/z apart indicate the polymeric nature of the compounds.

What's inside

• Introduction
• HPLC Conditions
• MS Parameters
• Instrumentation
• TurboDDS Parameters
• Results & Discussion
• Conclusion

Frequently asked questions

What is the purpose of this application note?

This document describes a method to separate and analyze the components of the Ethomeen T/20H emulsifier using LC/MS.

What instrument is used for the analysis?

The analysis uses a Varian 500-MS Ion Trap LC/MS equipped with an ESI source.

How much sample is injected for the test?

The procedure requires an injection volume of 100 pL.