Agilent 1290 Infinity LC Rapid Gradient Elevated Temperature UHPLC of Flavonoids Application Note

About this manual

This document details a method for analyzing naringin and other flavonoids in citrus fruits (grapefruit, orange, lime) using Ultra-High Performance Liquid Chromatography (UHPLC). The study utilizes an Agilent 1290 Infinity LC System equipped with ZORBAX RRHD Eclipse Plus C18 columns to resolve over 70 peaks in grapefruit juice within seven minutes.

The application note highlights the benefits of operating at high flow rates (0.8 mL/min) and elevated temperatures (40°C or 90°C). These conditions significantly reduce column re-equilibration time compared to conventional HPLC, thereby increasing overall lab productivity without sacrificing resolution. The document includes experimental procedures, chromatographic results for various citrus samples, and calibration curves.

What's inside

• Abstract
• Introduction
• Experimental
• Results and Discussion
• Chromatograms

Frequently asked questions

What is the maximum flow rate used for column re-equilibration in this method?

The method uses a high flow rate of 0.8 mL/min to reduce re-equilibration time.

What is the recommended column temperature for this analysis?

The analysis can be performed at either 40°C or 90°C.

How much ground rind sample is required for extraction?

Two hundred milligrams of ground rind are extracted with 10 mL MeOH.