A high throughput method for the separation of 16 polycyclic aromatic hydrocarbons (PAHs) using the Agilent 1290 Infinity LC system was developed
This document presents a high-throughput method for the separation of 16 polycyclic aromatic hydrocarbons (PAHs). The method was developed using the Agilent 1290 Infinity LC system and a ZORBAX Eclipse PAH column. The note details two optimized methods: one using acetonitrile as the mobile phase modifier for separation in less than 3 minutes, and another using methanol for separation in 6 minutes. Both methods are compared regarding analysis time and solvent volume consumption.
| Column Dimensions | 2.1 X 50 mm |
|---|---|
| Column Particle Size | 1.8 pm |
| Analysis Time (Acetonitrile) | less than 3 min |
| Analysis Time (Methanol) | 6 min |
| Flow Rate (Acetonitrile) | 0.9 mL/min |
| Flow Rate (Methanol) | 0.75 mL/min |
| Detection Wavelength | 254 nm |
| Injection Volume | 1 pL |
The separation of 16 EPA PAHs using acetonitrile as the organic modifier is achieved in less than 3 minutes.
Using methanol as the organic modifier in the mobile phase allows for the baseline separation of 16 EPA PAHs within 6 minutes.
The method utilizes an Agilent ZORBAX Eclipse PAH column with dimensions of 2.1 x 50 mm and a particle size of 1.8 pm.