This Application Note describes the separation of nine food additives, acesulfame K, aspartame, caffeine, benzoic acid, sorbic acid, saccharine, trans-cinnamic acid and vanillin using the Agilent 1290 Infinity LC system. The ability of the Agilent 1290 Infinity LC pump to deliver mobile phases at high pressures of up to 1200 bars enables separation of the nine selected additives within a run time of 1 min. The Agilent 1290 Infinity Diode Array Detector (DAD) equipped with Agilent’s proprietary Max-Light flow cell provides sufficient sensitivity for reliable analysis of even 1 µL of the sample. The developed method was applied for the screening of additives present in soft drinks and colas.
This document details a method for the separation and analysis of nine food additives, including sweeteners, preservatives, and flavorants, using the Agilent 1290 Infinity LC system. The method utilizes the system's high-pressure capabilities (up to 1200 bars) and Rapid Resolution High Definition (RRHD) columns to achieve separations within a 1-minute run time.
| Maximum Pressure | 1200 bars |
|---|---|
| Run Time | 1 min |
| Injection Volume | 1 pL |
| Detection Wavelength | 210 nm |
The separation of the nine selected additives is achieved within a run time of 1 min.
The Agilent 1290 Infinity Diode Array Detector (DAD) equipped with the Max-Light flow cell is used.
The Agilent 1290 Infinity LC pump can deliver mobile phases at high pressures of up to 1200 bars.