Agilent 1290 Infinity LC System Analysis of TCM Injections Application Note

About this manual

The document details an ultra-high performance liquid chromatography (UHPLC) method developed to analyze Traditional Chinese Medicine (TCM) injections, specifically Shenmai injection. The method utilizes a hybrid column setup connecting two Agilent ZORBAX columns to achieve good separation of complex components like ginsenosides and saponins.

What's inside

• Introduction
• Experimental Equipment
• Sample Preparation
• Chromatographic Conditions
• Results and Discussion
• Column Optimization

Frequently asked questions

What is the total analysis time for the Shenmai injection method?

The developed method requires only 11 minutes to complete the analysis.

At what temperature should the column be maintained during analysis?

The column temperature must be set to 55 °C for the analysis.

What is the flow rate used for the chromatographic separation?

The flow rate is set to 0.6 mL/min to leverage fast speed.