Agilent Quantification of Alprazolam in Dried Blood Spots Using Agilent 1290 Infinity LC System and 6460 Triple Quadrupo

About this manual

The document presents a validated method to quantitatively determine alprazolam concentrations in dried whole rat blood spots. The procedure involves spotting blood onto FTA Elute MicroCards, punching cores, and extracting analytes using ultrasonication with acetonitrile/water containing formic acid.

Chromatographic separation is achieved on an Agilent ZORBAX Eclipse Plus RRHD C18 column using two different flow rates (1.0 mL/min or 1.8 mL/min) to optimize run time and sensitivity. The method utilizes multiple reaction monitoring (MRM) in positive ion mode for detection, achieving high accuracy and precision within the validated concentration ranges.

What's inside

• Abstract
• Instrument conditions
• LC/MS/MS system components
• Mobile phase preparation
• Gradient parameters
• Mass spectrometer settings
• Method validation results
• Calibration curves
• Sensitivity and accuracy data

Frequently asked questions

What column is used for the alprazolam quantification method?

The method uses an Agilent ZORBAX Eclipse Plus RRHD C18, 2.1 x 50 mm, 1.8 pm column.

What is the lower limit of quantification (LLOQ) for Method 1?

The LLOQ for Method 1 is 0.15 ng/mL.

What flow rates are tested in the application?

The method was validated at flow rates of 1.0 mL/min and 1.8 mL/min.