This Application Note demonstrates that stringent sensitivity requirements for the detection of potentially harmful primary aromatic amines can be fulfilled when using an Agilent 1290 Infinity LC System equipped with the 1290 large volume injection kit. This enables the Agilent 1290 Infinity Autosampler for injection volumes up to 120 μL for sample enrichment on the column. A high sensitivity 60-mm Max-Light flow cell in the Agilent 1290 Infinity Diode Array Detector gives additional detection sensitivity.
This document describes an analytical method developed on the Agilent 1290 Infinity LC System for the detection and quantification of primary aromatic amines (PAAs) in consumer products like printed paper and plastics. The method addresses legal requirements to detect these potentially harmful compounds at levels as low as 1 ppb.
The system enables injection volumes up to 120 pL for sample enrichment on the column.
The limit of detection was found at 0.5 ppb.
A concentration level of 2 ng/mL was defined as the limit of quantification.