Update: 30 September, 2023
This Application Note describes the separation of a trypsin-digested protein sample, using the Agilent 1290 Infinity LC System with Agilent ZORBAX Eclipse Plus C18, 150 mm × 2.1 mm, 1.8 µm particles column. Peak capacity variation with change in flow rate and time for various gradients were evaluated. Increase in flow rate from 0.2mL/min to 1.2mL/min effectively improved the peak capacity value to double and increase in gradient time from 5minutes to 50 minutes enhanced a 3.4 fold improve- ment in peak capacity value. The unique design of the Agilent 1290 Infinity LC System tolerates 1200 bar column back pressure, allowing higher flow rates, and reducing the run time.
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MD5 Checksum: 54668DE3FCAE6EE7AC2F1B105002F8BB
Publication date: 12 June, 2012
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