Agilent 1290 Infinity LC Application Note: High Sensitivity UHPLC-DAD Analysis of Azo Dyes

This document describes the analysis of azo dyes using the Agilent 1290 Infinity LC System and the 60 mm Max-Light high sensitivity flow cell. The performance of the high sensitivity method is investigated by comparing the results with those obtained using a standard flow cell.


Agilent 1290 Infinity LC Application Note: High Sensitivity UHPLC-DAD Analysis of Azo Dyes - cover page
Brand
Agilent
Category
Other
Document type
Application Note
Language
English
Pages
8
File format
PDF
File size
737 KB
Published
11 June, 2012
Updated
29 September, 2023
MD5 checksum
80E2D5711F88CC2F28DC64CF758E9530

About this manual

This document describes a method for analyzing toxic aromatic amines released from banned azo colorants using the Agilent 1290 Infinity LC System. The study compares the performance of a standard flow cell (10 mm optical path length) against the Agilent Max-Light high sensitivity flow cell (60 mm optical path length).

What's inside

  • Introduction
  • Experimental
  • Results and discussion
  • Equipment
  • Chromatographic Conditions
  • Conclusion
  • References

Specifications

Optical path length (High Sensitivity Cell)60 mm
Optical path length (Standard Cell)10 mm
Flow rate1.6 mL/min
Detection limits0.2-1 ng/mL

Frequently asked questions

What is the benefit of the high sensitivity flow cell?

The high sensitivity flow cell increases sensitivity by a factor of 5-6 compared to the standard cell.

Which amines are analyzed in this application note?

The note analyzes a set of 22 potentially carcinogenic amines derived from azo dyes.

Download PDF (737 KB)

More Agilent Manuals


View all Agilent manuals →