The qualitative and quantitative analysis of pesticides at trace levels in baby food matrices using UHPLC and triple quadrupole MS is demonstrated. Sample preparation is performed using an Agilent Bond Elut QuEChERS kit for extraction and dispersive SPE. The extracts are analyzed by LC/MS/MS on an Agilent 1290 Infinity LC system coupled to an Agilent 6460 triple quadrupole LC/MS using Dynamic MRM. The method and extraction performance were evaluated in terms of repeatability, linearity and sensitivity. Moreover the influence of the additional dispersive SPE cleanup was investigated. Detection limits were between 500 ng/kg and 10 ng/kg (ppt), which is much lower than the maximum residue level (MRL) of 10 µg/kg (ppb) imposed by the European Union.
This document details a method for the qualitative and quantitative analysis of 40 pesticides at trace levels in baby food matrices. The method utilizes UHPLC and triple quadrupole mass spectrometry with Dynamic MRM to achieve detection limits between 500 ng/kg and 10 ng/kg.
Sample preparation involves extraction using an Agilent Bond Elut QUEChERS kit followed by dispersive SPE cleanup. The analysis is performed on an Agilent 1290 Infinity LC system coupled to an Agilent 6460 triple quadrupole LC/MS using Dynamic MRM with a total analysis time of 10 minutes.
| Total analysis time | 10 min |
|---|---|
| Detection limits | 500 ng/kg and 10 ng/kg |
| Column | Agilent ZORBAX Eclipse Plus RRHD C18 |
| Ionization mode | Electrospray, positive ionization |
| Drying gas temperature | 250 °C |
| Capillary voltage | 4500 V |
| Cycle time | 200 ms |
The total analysis time is 10 minutes, including re-equilibration.
An Agilent Bond Elut QUEChERS kit is used for extraction and dispersive SPE.
Detection limits were between 500 ng/kg and 10 ng/kg.
The method analyzes 40 pesticides in baby food.