This Application Note describes the development of a fast method for the determination of 13 aldehyde and ketone derivates with the Agilent 1290 Infinity LC system. The method, which used acetone as organic co-solvent separates the analytes within 3.5 minutes.
This document details the development of a high-throughput method to separate 13 aldehyde and ketone derivatives within 3.5 minutes. The method utilizes the Agilent 1290 Infinity LC system, specifically leveraging its small dwell volume of 125 pL to enable fast separations with narrow bore columns. Acetone was used as the organic co-solvent in the mobile phase.
| Dwell volume | 125 pL |
|---|---|
| Maximum pressure | 1200 bar |
| Analysis time | 3.5 minutes |
| Flow rate | 1.2 mL/min |
| Detection wavelength | 360 nm |
| Column dimensions | 50 mm x 2.1 mm, 1.8 pm |
The separation of 13 aldehyde and ketone derivatives is completed in around 3.5 minutes.
Acetone was used as the organic co-solvent.
The critical resolution is 1.6 between peak pair 6 and 7.